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Title:Biochemical Analysis of the Molecular Factors of Leucyl -Trna Synthetase That Ensure Aminoacylation Fidelity
Author(s):Hellmann, Rachel Alice
Doctoral Committee Chair(s):Martinis, Susan A.
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:The canonical genetic code has potentiel to be expanded by developing orthogonal aaRS:tRNA systems. Tools to generate an orthogonal LeuRSaRNA Leu pair were developed by using a two plasmid system in an E. coli strain containing a nonfunctional genomic lacZ gene with an amber stop codon. The plasmid pRAH curies tRNALeu with an amber suppressor anticodon, while pKEG harbors LeuRS. From this system, directed evolution approaches can be used to screen for an orthogonal tRNA and a LeuRS derivative that will charge it.
Issue Date:2009
Type:Text
Language:English
Description:160 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009.
URI:http://hdl.handle.net/2142/84863
Other Identifier(s):(MiAaPQ)AAI3362919
Date Available in IDEALS:2015-09-25
Date Deposited:2009


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