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Title:The Use of High-Affinity T Cell Receptors to Study Specificity in the 2C T Cell System
Author(s):Bowerman, Natalie A.
Doctoral Committee Chair(s):Kranz, David M.
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Health Sciences, Immunology
Abstract:Finally, soluble high-affinity TCRs are promising reagents for the detection and diagnosis of specific virus or tumor antigens (peptide-MHC) on the cell surface. In addition, the TCRs would provide a useful probe for tracking the processing and presentation of specific antigens. Chapter 6 discusses the development of soluble single-chain TCRs with enhanced properties, such as increased avidity and signal amplification, to detect antigen. The results show that tetrameric and multi-fluorophore labeled forms of the high-affinity 2C TCR called m67 were capable of detecting low levels of specific peptide-MHC on the surface of both tumor cells and when the antigen was cross-presented on stromal cells in a solid tumor.
Issue Date:2009
Type:Text
Language:English
Description:150 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009.
URI:http://hdl.handle.net/2142/84864
Other Identifier(s):(MiAaPQ)AAI3391888
Date Available in IDEALS:2015-09-25
Date Deposited:2009


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