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Title:T Cell Receptor Alpha Chain Residues Involved in Antibody Binding and Recognition of peptide/MHC Ligand
Author(s):Brodnicki, Thomas Charles
Doctoral Committee Chair(s):Kranz, David M.
Department / Program:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Health Sciences, Immunology
Abstract:Mutant 2C scTCRs were further examined in ligand binding experiments. The 2C TCR recognizes a peptide (QL9) presented by the MHC product L$\sp{\rm d}$. Four alanine mutations which affected mAb binding also disrupted binding to the QL9/L$\sp{\rm d}$ complex by greater than ten-fold relative to the wild-type scTCR. A fifth $\alpha$ chain mutation, located within a loop that is analogous to the fourth hypervariable region (HV4) of the $\beta$ chain, disrupted binding to QL9/L$\sp{\rm d}$ approximately five-fold. Based on these results and a homology model of the 2C $\alpha$ chain variable region, these five residues are likely to be involved in either stabilization of CDR loops or in direct contact with the peptide/MHC ligand.
Issue Date:1997
Description:195 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997.
Other Identifier(s):(MiAaPQ)AAI9717257
Date Available in IDEALS:2015-09-25
Date Deposited:1997

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