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Title:Essential Glycosyltransferases in Schizosaccharomyces Pombe
Author(s):Colussi, Paul Attilio
Doctoral Committee Chair(s):Peter A.B.Orlean
Department / Program:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Microbiology
Abstract:I cloned cDNAs encoding S. pombe, human and Caenorhabditis briggsiae Dol-P-Man synthases, all three of which lack a characteristic carboxy-terminal transmembrane region found on previously isolated Dol-P-Man synthases. Known Dol-P-Man synthases can therefore be divided into a 'human' or 'S. cerevisiae' class, although the two are functionally equivalent for I showed that the S. cerevisiae DPM1 gene and its human counterpart both complement the lethal dpm1$\sp+$ null mutation in S. pombe. The 'human' class of Dol-P-Man synthases, however, do not complement an S. cerevisiae dpm1 null mutant, and, unlike the S. cerevisiae class of enzymes, do not confer Dol-P-Man synthetic activity on E. coli cells. I propose that the 'human' class of Dol-P-Man synthase requires one or more auxiliary proteins for catalytic activity or to mediate membrane association. The facts that Dol-P-Man synthase is an essential enzyme in yeast, and that the Ustilago and Trypanosoma synthases are in a different class from the human enzyme, raises the possibility that Dol-P-Man synthase could be exploited as a target for selective inhibitors of pathogenic eukaryotic microbes.
Issue Date:1997
Description:147 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997.
Other Identifier(s):(MiAaPQ)AAI9737080
Date Available in IDEALS:2015-09-25
Date Deposited:1997

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