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Title:Human Estrogen Receptor(alpha) Activity on Estrogen Responsive, Integrated Chloramphenicol Acetyltransferase (Cat) Reporter Constructs in Chinese Hamster Ovary (Cho) Cells
Author(s):Eicken, Elizabeth Jean
Doctoral Committee Chair(s):Shapiro, David J.
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Molecular
Abstract:The estrogen receptors endogenous to CHO cells did not induce transcription of chromosomal copies of the more complex 2ERETKCAT promoter. In transient transfections hER$\alpha$ regulated transcription of the three 2ERETKCAT cell lines. We found that an ER mutant containing only AF1 elicited a dose dependent activation of the 2ERETKCAT promoter. This represents the first report of activation of an integrated reporter gene by this activation function.
Issue Date:1998
Type:Text
Language:English
Description:134 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998.
URI:http://hdl.handle.net/2142/84890
Other Identifier(s):(MiAaPQ)AAI9834670
Date Available in IDEALS:2015-09-25
Date Deposited:1998


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