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Title:The Estrogen Response Element and the KRAB Repression Domain as Modulators of Estrogen Receptor Action
Author(s):De Haan, Georgius
Doctoral Committee Chair(s):Shapiro, David J.
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:Repressing expression of estrogen regulated genes would greatly facilitate studies of their function. Therefore we transformed ER into a transcription repressor by fusing the KRAB transcription repressor domain of the Kox1 protein to ER and various ER mutants. Incorporation of DBD mutations conferring increased ERE affinity previously identified in a genetic selection, allowed us to effectively repress in a ligand dependent fashion both basal and estrogen receptor induced transcription from a promoter containing a single non consensus ERE. These ER-KRAB chimeras therefore constitute powerful tools for the study of estrogen regulated gene expression.
Issue Date:1999
Type:Text
Language:English
Description:143 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999.
URI:http://hdl.handle.net/2142/84908
Other Identifier(s):(MiAaPQ)AAI9953002
Date Available in IDEALS:2015-09-25
Date Deposited:1999


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