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Title:DNA and Ligand Binding and Kinetic Properties of Wild-Type and Mutant RsrI [n6-Adenine] DNA Methyltransferases
Author(s):Szegedi, Sandra Suzanne
Doctoral Committee Chair(s):Gumport, R.I.
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:To complement the biochemical characterization of M•Rsr I in vitro, DNA binding analysis in vivo was performed using the bacteriophage P22-based challenge-phage assay. I isolated a catalytically impaired mutant, M•RsrI (L72P), that, unlike wild-type M•RsrI, binds in a methylation-sensitive, site-specific manner to DNA.
Issue Date:2000
Type:Text
Language:English
Description:183 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000.
URI:http://hdl.handle.net/2142/84915
Other Identifier(s):(MiAaPQ)AAI9971200
Date Available in IDEALS:2015-09-25
Date Deposited:2000


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