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Title:Modulation of the Expression of Multidrug Resistance Proteins by Sulforaphane and Erucin
Author(s):Harris, Kristin E.
Doctoral Committee Chair(s):Elizabeth Jeffery
Department / Program:Nutritional Sciences
Discipline:Nutritional Sciences
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Health Sciences, Nutrition
Abstract:Sulforaphane increases gene expression through binding of the Nrf2 transcription factor to the antioxidant response element (ARE) in the promoter region of target genes. Because SF and ER increased MRP1 expression at the mRNA level, we investigated the impact of these compounds on MRP1 transcription. Non-small cell lung cancer cells (A549) and human liver carcinoma cells (HepG2) were transfected with an MRP1-luciferase reporter gene and treated with SF or ER. Neither SF nor ER increased luciferase activity in the transfected cells. In addition, overexpression of Nrf2 in A549 cells had no effect on MRP1-luc expression. These results indicate that SF and ER increase MPR1 mRNA and protein levels via a non-transcriptional mechanism and that activation of Nrf2 does not induce transcription of the mrp1 gene.
Issue Date:2007
Type:Text
Language:English
Description:116 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007.
URI:http://hdl.handle.net/2142/84951
Other Identifier(s):(MiAaPQ)AAI3269911
Date Available in IDEALS:2015-09-25
Date Deposited:2007


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