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Title:Effects of Heterodera Glycines Population Densities on Fusarium Solani F. Sp. Glycines Colonization of Soybean Roots and Development of Sudden Death Syndrome
Author(s):Jackson, Tamra A.
Doctoral Committee Chair(s):Niblack, Terry L.
Department / Program:Crop Sciences
Discipline:Crop Sciences
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Microbiology
Abstract:Estimation of root colonization by Fsg with the plating bioassay was difficult and the results were often unsatisfactory in these experiments. Therefore, an alternative method was explored, relative quantification with real-time quantitative PCR (QPCR), to estimate fungal colonization of soybean roots. QPCR was more sensitive than the plating bioassays at detecting the fungus: Fsg DNA was detected in 371 out of 372 root samples with QPCR, while CFU were only detected in 14.5% of the samples with the plating bioassay. The plating bioassay required at least 10 days longer to complete and the results had higher variability than those obtained with QPCR. Overall, QPCR had many advantages over the plating bioassay and has the potential for several more practical applications.
Issue Date:2005
Type:Text
Language:English
Description:128 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.
URI:http://hdl.handle.net/2142/85020
Other Identifier(s):(MiAaPQ)AAI3182286
Date Available in IDEALS:2015-09-25
Date Deposited:2005


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