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Title:Modification of Vegetable Soybean Sugar Content via Gene Transformation
Author(s):Chanprame, Sermsiri
Doctoral Committee Chair(s):Widholm, Jack M.
Department / Program:Crop Sciences
Discipline:Crop Sciences
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Agriculture, Plant Culture
Abstract:To modify soybean sugar content, somatic embryos of soybean cvs. Peterson Jade, Daelip Hakja (PI 157.419), Yakumo Meaka (PI 417.468), Kinshu (PI 549.065) Kaohsiung No.1 (PI 536.546) and Jack were transformed with yeast invertase (SUC 2) and $\alpha$-galactosidase (agl A) from Aspergillus niger under the control of the lectin promoter using particle bombardment. The transformation was not very successful. In some cultivars, no green clumps were obtained after 8 weeks of selection in FG medium containing 25 $\mu$g/ml hygromycin B. The green clumps obtained from transformation and selection were subjected to PCR analysis for the transgenes. Some lines were PCR positive for the hygromycin resistance and/or $\alpha$-galactosidase genes but Southern and northern analysis gave negative results. These results suggest that the transformed genes were lost during the somatic embryo multiplication due to the friability of some green clumps that were discarded during the culture process.
Issue Date:1998
Description:97 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998.
Other Identifier(s):(MiAaPQ)AAI9834660
Date Available in IDEALS:2015-09-25
Date Deposited:1998

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