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Title:Junction Specificity and Transmembrane Linkages of Integrins
Author(s):McDonald, Kevin Alan
Doctoral Committee Chair(s):Horwitz, Alan F.
Department / Program:Biology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Molecular
Abstract:We have also isolated a monoclonal antibody, P4B2, which localizes to multiple adhesion junctions, namely, a subset of focal adhesions, the Z-disc of muscle, and neuromuscular junctions. Immunopurification of the antigen to this antibody from chicken brain tissue yielded a complex of three prominent proteins with mobilities of 36, 30 and 18 kD. Amino acid sequencing of the purified proteins identified the 36 kD protein as GAPDH. The other 2 protein bands were heterogeneous, containing proteins found in the synaptic vesicle fusion core complex. Immunolocalization of P4B2 antigen in developing cultured muscle cells showed that the antigen is incorporated into Z-lines soon after the sarcomeric architecture was positive for $\alpha$-actinin. Together, the data indicate the P4B2 antigen is part of a unique GAPDH containing protein complex that may be involved in reinforcement of established cytoskeletal structures.
Issue Date:1997
Description:158 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997.
Other Identifier(s):(MiAaPQ)AAI9737192
Date Available in IDEALS:2015-09-25
Date Deposited:1997

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