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Title:Exploring the Quinol Oxidation Mechanism at the Qo-Site of Bc1 Complex Through Site-Directed Mutagenesis
Author(s):Lhee, Sangmoon
Doctoral Committee Chair(s):Crofts, Antony R.
Department / Program:Biophysics and Computational Biology
Discipline:Biophysics and Computational Biology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Microbiology
Abstract:The backbone amide groups of FL132 and FC134 give additional electron withdrawing force to the Rieske-type [2Fe-2S] cluster and the residues in the 132-134 positions play roles in anchoring the ISP to the surface of the Qo-pocket. FG133 has unique ϕ/psi angles that restrict the positions of two adjacent residues, FL132 and FC134, determining the favorable hydrogen bonds to the S2 of the cluster. A preliminary X-ray crystallographic study of FG133S showed the alterations in the hydrogen bond pattern surrounding the cluster and the flexible disulfide bridge between FC134 and FC151 by changing the ϕ/psi angles of FG133. Several mutant ISP's at FG133 and FL132 were prepared for investigating the flexibility in the backbone structure and the modifications in electrochemical parameters.
Issue Date:2007
Type:Text
Language:English
Description:129 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007.
URI:http://hdl.handle.net/2142/85465
Other Identifier(s):(MiAaPQ)AAI3301179
Date Available in IDEALS:2015-09-25
Date Deposited:2007


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