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Title:Fluorescent Tools for Watching Conformational Changes of Biological Molecules
Author(s):Toprak, Erdal
Doctoral Committee Chair(s):Selvin, Paul R.
Department / Program:Biophysics and Computational Biology
Discipline:Biophysics and Computational Biology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biophysics, General
Abstract:Finally, we used a single molecule fluorescence quenching assay to study the timing of the neck linker conformational change that occurs as kinesin walks processively along a microtubule. We observed repetitive, alternating cycles of fluorescence and quenching as individual kinesin motors walked along microtubules. By monitoring the position of the kinesin during these fluorescence cycles, we were able to conclude that one cycle of quenching/unquenching occurs with each 8 nm step, and that each step corresponds to an ATP-induced separation of neck linkers.
Issue Date:2007
Description:135 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007.
Other Identifier(s):(MiAaPQ)AAI3301237
Date Available in IDEALS:2015-09-25
Date Deposited:2007

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