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 Title: Electron Transfer on the Donor and Acceptor Sides of Photosystem II Explored by Site-Directed Mutagenesis and Kinetic Spectroscopy Author(s): Kanazawa, Atsuko Doctoral Committee Chair(s): Crofts, Antony R. Department / Program: Biophysics and Computational Biology Discipline: Biophysics and Computational Biology Degree Granting Institution: University of Illinois at Urbana-Champaign Degree: Ph.D. Genre: Dissertation Subject(s): Biology, Plant Physiology Abstract: H252Q and six donor-side mutants became the important tools to investigate a new model of neutral tyrosine radical, $\rm Y\sb{Z}\sp\cdot,$ introduced by Gilchrist et al. (1995) and Hoganson et al. (1995). In this new model, $\rm Y\sb{Z}$ is not simply a redox active residue in the electron transfer reactions, but is also directly involved in the water-oxidation. The models are critically-dependent on the presence of a neutral radical form of $\rm Y\sb{Z}\ (Y\sb{Z}\sp{\cdot}).$ The donor-side mutants, $\rm E189 > L,Q,$ and $\rm N191 > A,D,L,$ had only small effects on the pH-dependence of the rate of Q$\rm\sb{A}\sp-$ and oxidized $\rm Y\sb{Z}$ recombination. On the other hand, E189D mutant showed a pH-independent in rate of $\rm Q\sb{A}\sp-$ and oxidized $\rm Y\sb{Z}$ recombination. Based on these data, a simple thermodynamic model was introduced to explain the results and the model of $\rm Y\sb{Z}\sp{\cdot}$ as an oxidized form. Issue Date: 1997 Type: Text Language: English Description: 174 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997. URI: http://hdl.handle.net/2142/85481 Other Identifier(s): (MiAaPQ)AAI9812648 Date Available in IDEALS: 2015-09-25 Date Deposited: 1997
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