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Title: | Electron Transfer on the Donor and Acceptor Sides of Photosystem II Explored by Site-Directed Mutagenesis and Kinetic Spectroscopy |
Author(s): | Kanazawa, Atsuko |
Doctoral Committee Chair(s): | Crofts, Antony R. |
Department / Program: | Biophysics and Computational Biology |
Discipline: | Biophysics and Computational Biology |
Degree Granting Institution: | University of Illinois at Urbana-Champaign |
Degree: | Ph.D. |
Genre: | Dissertation |
Subject(s): | Biology, Plant Physiology |
Abstract: | H252Q and six donor-side mutants became the important tools to investigate a new model of neutral tyrosine radical, $\rm Y\sb{Z}\sp\cdot,$ introduced by Gilchrist et al. (1995) and Hoganson et al. (1995). In this new model, $\rm Y\sb{Z}$ is not simply a redox active residue in the electron transfer reactions, but is also directly involved in the water-oxidation. The models are critically-dependent on the presence of a neutral radical form of $\rm Y\sb{Z}\ (Y\sb{Z}\sp{\cdot}).$ The donor-side mutants, $\rm E189 > L,Q,$ and $\rm N191 > A,D,L,$ had only small effects on the pH-dependence of the rate of Q$\rm\sb{A}\sp-$ and oxidized $\rm Y\sb{Z}$ recombination. On the other hand, E189D mutant showed a pH-independent in rate of $\rm Q\sb{A}\sp-$ and oxidized $\rm Y\sb{Z}$ recombination. Based on these data, a simple thermodynamic model was introduced to explain the results and the model of $\rm Y\sb{Z}\sp{\cdot}$ as an oxidized form. |
Issue Date: | 1997 |
Type: | Text |
Language: | English |
Description: | 174 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997. |
URI: | http://hdl.handle.net/2142/85481 |
Other Identifier(s): | (MiAaPQ)AAI9812648 |
Date Available in IDEALS: | 2015-09-25 |
Date Deposited: | 1997 |
This item appears in the following Collection(s)
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Dissertations - Biophysics and Computational Biology
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Graduate Dissertations and Theses at Illinois
Graduate Theses and Dissertations at Illinois