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Title:In Vivo Fluorometric Assessment of the Skin Microcirculation
Author(s):Oh, Deborah Kyung
Doctoral Committee Chair(s):Richard L. Magin
Department / Program:Biophysics and Computational Biology
Discipline:Biophysics and Computational Biology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Engineering, Biomedical
Abstract:A tissue uptake model was developed to describe the fluorescein dermofluorometer signal in terms of the local blood flow and capillary permeability. The fluorescein signal is characterized by a wash-in time constant dependent on both the blood flow and permeability. Under flow-limited conditions the wash-in time constant is inversely proportional to the blood flow only. Significant differences in the fluorescein wash-in time constant were measured between diabetic and non-diabetic subjects (p < 0.10) and between diabetic patients with chronic foot ulcers and those without ulcers (p < 0.01). A new technique, dual dye dermofluorometry, which measures the simultaneous signals from an intravascular tracer, indocyanine green, and a diffusible tracer, sodium fluorescein, was developed. The indocyanine green signal is described by a physiologically-based pharmacokinetic model with a wash-in time constant inversely proportional to the blood flow. The extravascular fluorescein signal is extracted from the observed signal by subtracting a scaled version of the indocyanine green signal. Indocyanine green wash-in time constants were significantly different between patients whose foot ulcers led to amputation relative to those whose foot ulcers healed without surgery (p < 0.10) and between patients with past foot ulcers relative to patients with no history of foot ulcers (p < 0.05). It was determined that while the flow-limited assumption for fluorescein was valid in healthy rat skin, it was not valid in diabetic subjects.
Issue Date:1999
Type:Text
Language:English
Description:157 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999.
URI:http://hdl.handle.net/2142/85491
Other Identifier(s):(MiAaPQ)AAI9944955
Date Available in IDEALS:2015-09-25
Date Deposited:1999


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