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Title:Imaging Neuromuscular Synaptogenesis
Author(s):Carrero-Martinez, Franklin Alexander
Doctoral Committee Chair(s):Chiba, Akira
Department / Program:Cell and Developmental Biology
Discipline:Cell and Developmental Biology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Cell
Abstract:Successful synaptogenesis requires collaboration of cellular and molecular dynamics. Whereas synaptic matchmaking relies on cell-specific recognition molecules, subsequent synaptogenesis requires proteins that are common to a given synaptic type. Biochemical studies reveal that PSD-95/Dlg family scaffolding proteins could coordinate postsynaptic molecular assembly at glutamatergic synapses. Genetic studies demonstrate their role during synaptic maintenance and plasticity. However, it is unclear whether initial synaptogenesis requires PSD-95/Dlg and, if so, how its function collaborates with cell-specific matchmaking that precedes synaptogenesis. Here, using whole-mount high-resolution live imaging, we show that glutamatergic synaptogenesis on all muscles in Drosophila embryos is preceded by formation of a myopodial cluster into which PSD-95/Dlg is rapidly recruited. Cell-specific disruption of myopodial clustering affect synaptogenesis, while disrupting postsynaptic PSD-95/Dlg functions during this period aborts synaptogenesis without affecting myopodial cluster formation. These results support essential functions of postsynaptic PSD-95/Dlg and its collaboration with myopodial cluster at the onset of glutamatergic neuromuscular synaptogenesis.
Issue Date:2005
Description:131 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.
Other Identifier(s):(MiAaPQ)AAI3202069
Date Available in IDEALS:2015-09-28
Date Deposited:2005

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