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Title:Regulation of Gene Transcription by Estrogen Receptor Alpha: The Role of Cis Elements and Trans -Acting Factors
Author(s):Creekmore, Amy
Doctoral Committee Chair(s):Clayton, David F.; Nardulli, Ann M.
Department / Program:Cell and Developmental Biology
Discipline:Cell and Developmental Biology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Cell
Abstract:The differential recruitment of coregulatory proteins to DNA-bound ERalpha plays a critical role in mediating estrogen-responsive gene expression. We previously isolated and identified retinoblastoma-associated proteins 46 (RbAp46) and 48 (RbAp48), which are associated with chromatin remodeling, histone deacetylation, and transcription repression as proteins associated with the DNA-bound ERalpha. We have demonstrated that RbAp46 and RbAp48 interact with ERalpha in vitro and in vivo, associate with ERalpha at endogenous estrogen-responsive genes, and alter expression of endogenous ERalpha-activated and -repressed genes in MCF-7 breast cancer cells. Our findings reveal that RbAp48 limits expression of estrogen-responsive genes and that RbAp46 attenuates and enhances estrogen responsiveness in a gene-specific manner. The ability of the transacting factors RbAp46 and RbAp48 to interact with ERalpha and influence its activity reveals yet another role for these multifunctional proteins in regulating gene expression.
Issue Date:2008
Description:69 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008.
Other Identifier(s):(MiAaPQ)AAI3314753
Date Available in IDEALS:2015-09-28
Date Deposited:2008

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