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Title:Molecular and Genetic Analysis of the Role of Ku in Silencing and Telomere Function in Saccharomyces Cerevisiae
Author(s):Vandre, Catherine
Doctoral Committee Chair(s):Belmont, Andrew S.
Department / Program:Cell and Developmental Biology
Discipline:Cell and Developmental Biology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Molecular
Abstract:The binding of Ku at the telomere is an integral part of maintaining the specialized chromatin structure required for genomic stability at the telomeres. To gain further insight into the signals and complexes involved in the multiple processes contributing to normal telomere function, we performed a Synthetic Genetic Array analysis focusing on genes with roles in silencing (YKU70, SIR1, SAS2, SAS5, SET1, ASF1) and telomere length regulation (YKU70, BDF1, ANC1, RAD50, TEL1, RAD52). Double mutant analysis revealed functional relationships between genes by generating a network of 241 synthetic interactions between 144 genes at 30°C and a network of 355 synthetic lethal interactions between 146 genes at 37°C. The genetic interactions highlighted the underappreciated role of chromatin remodeling proteins in normal telomere function. Additionally, a screen for suppressors of Ku senescence at 37°C identified a role for inositol phosphate signaling in telomere length regulation.
Issue Date:2008
Type:Text
Language:English
Description:237 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008.
URI:http://hdl.handle.net/2142/86323
Other Identifier(s):(MiAaPQ)AAI3347551
Date Available in IDEALS:2015-09-28
Date Deposited:2008


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