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Title:The Effects of Four Consecutive Days of Acute Exercise on Macrophage Antigen Presentation
Author(s):Ceddia, Michael Anthony
Doctoral Committee Chair(s):Woods, Jeffrey A.
Department / Program:Kinesiology and Community Health
Discipline:Kinesiology and Community Health
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Health Sciences, Recreation
Abstract:These studies determined the effects of exercise on the ability of M&phis;'s to present antigen to T cells. Pathogen-free male Balb/c mice (8 +/- 2 wk) were randomly assigned to either home cage control (HCC), moderate exercise (MOD, 18 m min-1, 5% grade, 0.5 hr day1), exhaustive exercise (EXH, 18--30 m min-1, 3 hr day), or treadmill control (TC, housed on treadmill for 3 hr but did not exercise) groups. The mice underwent treatments for a period of 4 days during peritoneal thioglycolate inflammation. Peritoneal M&phis;'s were harvested, purified, and incubated with chicken ovalburnin (C-OVA, 0--10 mg mL -1) for 18 hr. M&phis;'s were then co-cultured with C-OVA specific T cells for 48 hr at which time the supernatants were harvested and analyzed via ELISA for IL-2 as an indication of M&phis; AP. EXH exhibited suppressed (25--34 %) M&phis; AP across a wide range of C-OVA doses when measured immediately, 3, and 24 hr post-exercise. In contrast, MOD had reduced M&phis; AP only at 3 hr post-exercise. M&phis; AP was also lower in TC (4--27 %) when compared to HCC, but was significantly higher than EXH. The reduction in M&phis; AP was not due to exercise-induced differences in M&phis; number, percentage, expression of several accessory molecules (i.e., ICAM-1, B7-2 or MHC II), or in vitro production of M&phis; soluble factors (i.e., NO, PGE2, or TGF-beta). However, the exhaustive exercise-induced suppression did result from a reduced ability to generate antigenic peptides. This reduction in the ability of M&phis;s, from exhaustive exercised mice, to generate antigenic peptides may be mediated, in part by an increased lysosomal pH. In conclusion, the data lends evidence that may help explain the increased incidence of infection observed following prolonged exhaustive exercise or over-training.
Issue Date:1999
Type:Text
Language:English
Description:123 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999.
URI:http://hdl.handle.net/2142/86416
Other Identifier(s):(MiAaPQ)AAI9944812
Date Available in IDEALS:2015-09-28
Date Deposited:1999


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