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Title:The Integration and Excision Mechanisms of the Bacteroides Conjugative Transposon CTnDOT
Author(s):Cheng, Qi
Doctoral Committee Chair(s):Gardner, Jeffrey F.
Department / Program:Microbiology
Discipline:Microbiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Genetics
Abstract:A critical requirement for studying transposition is the availability of a defined in vitro transposition system. Using the partial-purified CTnDOT Int protein, an in vitro integration system was developed and a host factor(s) from Bacteroides cell extract was shown to be important for integration. The CTnDOT Int protein catalyzes the in vitro integration reactions by a coupling sequence mechanism similar to the in vivo reaction. Purified E. coli integration host factor (IHF) can substitute for the Bacteroides host factor(s) and significantly stimulates in vitro integration.
Issue Date:2002
Type:Text
Language:English
Description:152 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2002.
URI:http://hdl.handle.net/2142/86636
Other Identifier(s):(MiAaPQ)AAI3044068
Date Available in IDEALS:2015-09-28
Date Deposited:2002


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