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Title:Negative Regulation of the accBC Operon in Escherichia Coli
Author(s):James, Ethan Siddhartha
Doctoral Committee Chair(s):Cronan, John E., Jr
Department / Program:Microbiology
Discipline:Microbiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Molecular
Abstract:Expression of the accB gene from B. subtilis complemented E. coli AccB activity, but did not cause negative regulation of the accBC genes. A chimeric protein consisting of the amino-terminal 44 amino acids of E. coli AccB fused to the carboxyl-terminal biotinylation domain of B. subtilis AccB complemented AccB activity and caused negative regulation of the accBC operon. Complementation of ACC activity using the malonyl-CoA synthase gene (matB) from Rhizobium trifolii allowed the deletion of the accA and accB genes. Transcriptional activity of the accBC operon in this strain shows an absence of growth-rate dependent regulation.
Issue Date:2003
Type:Text
Language:English
Description:107 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003.
URI:http://hdl.handle.net/2142/86650
Other Identifier(s):(MiAaPQ)AAI3086089
Date Available in IDEALS:2015-09-28
Date Deposited:2003


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