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Title:Studies of Lipoic Acid Related Enzymes
Author(s):Jiang, Yanfang
Doctoral Committee Chair(s):Cronan, John E., Jr
Department / Program:Microbiology
Discipline:Microbiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Microbiology
Abstract:Using a similar strategy of library construction and screening, I also isolated the Vibrio harveyi acyl-ACP synthetase gene and showed that it encoded a 60.4 KDa protein that shared high homology with medium chain fatty acid-CoA ligases. I expressed the His-tagged protein at a high level in E. coli and purified it to homogeneity. To explore the physiological application of this enzyme in E. coli fatty acid metabolism, I constructed the first E. coli fabH (beta-ketoacyl-ACP synthetase III) bypass mutant in the presence of acyl-ACP synthetase and exogenous fatty acids.
Issue Date:2005
Type:Text
Language:English
Description:169 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.
URI:http://hdl.handle.net/2142/86677
Other Identifier(s):(MiAaPQ)AAI3182290
Date Available in IDEALS:2015-09-28
Date Deposited:2005


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