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Title:Engineering T Cell Receptors to Examine the Role of Affinity and *Kinetics in T Helper Cell Function
Author(s):Weber, K. Scott
Doctoral Committee Chair(s):Kranz, David M.
Department / Program:Microbiology
Discipline:Microbiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Health Sciences, Immunology
Abstract:Chapter five details the engineering of a class II restricted human TCR isolated from a patient with multiple sclerosis called 3A6. Yeast display of TCRs has previously relied on the use of an antibody specific for a properly folded TCR, here various strategies were used to generate stabilized 3A6 TCRs. Helpful strategies included: alteration of error-prone PCR dNTP concentrations, increasing induction media galactose concentrations, and generation of three-domain TCR constructs (Valpha-VbetaCbeta).
Issue Date:2005
Type:Text
Language:English
Description:180 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.
URI:http://hdl.handle.net/2142/86681
Other Identifier(s):(MiAaPQ)AAI3199171
Date Available in IDEALS:2015-09-28
Date Deposited:2005


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