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Title:Integration and Excision Mechanism of Bacteroides Conjugative Transposons
Author(s):Song, Bo
Doctoral Committee Chair(s):Abigail Salyers
Department / Program:Microbiology
Discipline:Microbiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Microbiology
Abstract:The third project is about the regulatory mechanism of RteB on CTnDOT. RteA and RteB are shown to be global regulators that regulate many genes on Bacteroides host. Whether RteB regulates CTnDOT and NBU1 excision directly or not is investigated in this study. Both RteB and the mutant are over-expressed in E. coli and partially purified. RteB is a soluble protein and gel shift result shows that RteB binds to 168bp upstream of rteC promoter region. However, the mutant did not bind to any probes tested. Footprint assay is required to determine the exact binding site on rteC promoter region.
Issue Date:2008
Type:Text
Language:English
Description:114 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008.
URI:http://hdl.handle.net/2142/86704
Other Identifier(s):(MiAaPQ)AAI3314901
Date Available in IDEALS:2015-09-28
Date Deposited:2008


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