Files in this item



application/pdf3395514.pdf (5MB)Restricted to U of Illinois
(no description provided)PDF


Title:Characterization of the Escherichia Coli Acyl Carrier Protein Phosphodiesterase
Author(s):Thomas, Jacob
Doctoral Committee Chair(s):Cronan, John E., Jr
Department / Program:Microbiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Microbiology
Abstract:The activity of AcpH was also examined in relation to the toxicity of N-pentylpantothenamide, an analog of the CoA precursor pantothenic acid. This analog is known to be a substrate for three CoA synthetic enzymes, resulting in a CoA analog with a metabolically inactive prosthetic group (ethyldethia-CoA) which is then transferred to ACP. It was thought that ethyldethia-ACP carrying this inactive prosthetic group was a poor substrate for AcpH, resulting in the selective turnover of holo-ACP. I have shown that this is not the case: ethyldethia-ACP is a substrate for AcpH and overexpression of this enzyme results in increased resistance to N-pentylpantothenamide. I have also shown that CoA synthesis is inhibited upon treatment with the analog and this is likely to be a major cause of its growth-inhibitory effect.
Issue Date:2009
Description:119 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009.
Other Identifier(s):(MiAaPQ)AAI3395514
Date Available in IDEALS:2015-09-28
Date Deposited:2009

This item appears in the following Collection(s)

Item Statistics