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Title:Investigation of Adenylate Kinases Isolated From Mesophilic and Thermophilic Members of the Archaeal Genus Methanococcus
Author(s):Haney, Paul Jeffrey
Doctoral Committee Chair(s):Konisky, Jordan
Department / Program:Microbiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Chemistry, Biochemistry
Abstract:Construction of chimeric proteins between M. voltae and M. jannaschii AKs along with site specific mutational analysis confirm the importance of specific stabilizing interactions identified by sequence comparisons, and allow the physical characteristic of these proteins to be significantly altered. Simple protein engineering increased the temperature optima of the Methanococcus voltae AK from 37$\sp\circ$C to 62$\sp\circ$C and its melting point from 68$\sp\circ$C to 89$\sp\circ$C while maintaining specific activity levels and approximately 90% of its original sequence. Analysis of the chimerical proteins also point strongly to the cooperative and non-additive nature of thermal stabilizing interactions within the methanococcal AKs. These studies also indicate that the molecular mechanisms which determine enzymatic temperature optima and overall protein stability are not directly related.
Issue Date:1997
Description:144 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997.
Other Identifier(s):(MiAaPQ)AAI9812611
Date Available in IDEALS:2015-09-28
Date Deposited:1997

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