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Title:The Roles of Peroxynitrite and Superoxide in Oxidative Damage to E. Coli
Author(s):Opperman, Kay Keyer
Doctoral Committee Chair(s):Imlay, James A.
Department / Program:Microbiology
Discipline:Microbiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:Peroxynitrite (ONOO$\sp{-}),$ a toxic biomolecule produced from the reaction of superoxide and nitric oxide (NO$\cdot)$ in the phagolysosome, may also be toxic by the same mechanism as O$\sb2{-}.$ Our in vivo results indicated that peroxynitrite has the same intracellular targets as superoxide. Of nineteen enzymes tested, only the dehydratase enzymes containing these iron-sulfur clusters were significantly affected by peroxynitrite challenge. These iron-sulfur clusters could be repaired, and both iron storage proteins and iron import seem to be necessary for efficient repair. This enzyme inactivation led to an increased intracellular free iron. However, unlike superoxide. the free iron was rapidly sequestered.
Issue Date:1997
Type:Text
Language:English
Description:156 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997.
URI:http://hdl.handle.net/2142/86724
Other Identifier(s):(MiAaPQ)AAI9812724
Date Available in IDEALS:2015-09-28
Date Deposited:1997


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