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Title:Proline Utilization in Salmonella Typhimurium: A Model for Regulation of Peripheral-Membrane Flavin Dehydrogenase-Lipid Interactions
Author(s):Surber, Mark William
Doctoral Committee Chair(s):Stanley Maloy
Department / Program:Microbiology
Discipline:Microbiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Genetics
Abstract:When it is located in the cytoplasm, PutA exists as a soluble dimer that is available to bind put operator DNA. However, proline oxidation and available membrane lipid allow PutA to undergo a dimer-to-monomer transition that is required for membrane association. On the other hand, if membrane lipid is not readily available PutA remains in the soluble, dimeric conformation and represses transcription of the put operon. Thus, although both proline and membrane lipid are required for induction of the put operon, a quaternary transition appears critical to prevent overexpression toxicity by this peripheral membrane flavin dehydrogenase.
Issue Date:1999
Type:Text
Language:English
Description:225 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999.
URI:http://hdl.handle.net/2142/86752
Other Identifier(s):(MiAaPQ)AAI9953152
Date Available in IDEALS:2015-09-28
Date Deposited:1999


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