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Title:Functional Analysis of the N -Terminal Transmembrane/signal -Anchor Sequence and Linker Domain of Cytochrome P450 2C2
Author(s):D., Balraj
Doctoral Committee Chair(s):Kemper, Byron W.
Department / Program:Microbiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Molecular
Abstract:Truncated forms of P450 2C2 lacking the N-terminal hydrophobic signal anchor sequence (Delta2- 20) or the signal anchor and linker region (Delta2-27) were expressed alone or as GFP fusions in E. coli and insect cells. The findings indicate that deletion of the signal anchor decreases assembly of P450 hemoprotein in E. coli but does not change the in vivo distribution of the protein. However, it alters the nature of membrane interaction such that P450 2C2 is no longer an integral membrane protein. Moreover, the results show that the linker domain is critical for the formation of functional P450 2C2 only when the P450 molecule is properly targeted and inserted in the membrane.
Issue Date:2000
Description:118 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000.
Other Identifier(s):(MiAaPQ)AAI9955603
Date Available in IDEALS:2015-09-28
Date Deposited:2000

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