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Title:Angiotensin II Modulation of NMDA Receptor Function: Characterization of Mechanisms Attenuating Excitotoxicity in Neurons
Author(s):Schelman, William Russell
Doctoral Committee Chair(s):Weyhenmeyer, James A.
Department / Program:Microbiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Molecular
Abstract:In the last study, we investigated the involvement of Bcl-2 in the suppression of NMDA-mediated neurotoxicity by Ang II. The viability of differentiated N1E and NG108 neuronal cell lines was reduced following exposure to NMDA in a dose-dependent manner. Excitotoxicity in N1E cells was inhibited by the noncompetitive NMDA receptor antagonist, MK-801. Further, NMDA receptor-mediated cell death was attenuated by Ang II in NG108 cells. A concentration-dependent decrease in intracellular Bcl-2 protein levels was observed following treatment with NMDA, and this reduction was inhibited by MK801. Addition of Ang II suppressed the NMDA receptor-mediated reduction in Bcl-2. The Ang II effect on NMDA-mediated changes in Bcl-2 expression was blocked by PD 123319, but was not significantly altered by losartan. Taken together, these results suggest that the decrease in Bcl-2 expression induced by NMDA is attenuated by AT2 activation. (Abstract shortened by UMI.).
Issue Date:2000
Description:138 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000.
Other Identifier(s):(MiAaPQ)AAI9971184
Date Available in IDEALS:2015-09-28
Date Deposited:2000

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