Characterization and Functional Analysis of the Arabidopsis Intron Splicing Factor ASF/SF2

Su, Chun-Lin

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Title:	Characterization and Functional Analysis of the Arabidopsis Intron Splicing Factor ASF/SF2
Author(s):	Su, Chun-Lin
Doctoral Committee Chair(s):	Schuler, Mary A.
Department / Program:	Plant Biology
Discipline:	Plant Biology
Degree Granting Institution:	University of Illinois at Urbana-Champaign
Degree:	Ph.D.
Genre:	Dissertation
Subject(s):	Biology, Molecular
Abstract:	ASF/SF2 protein is first time shown to be a potential substrate for the mitotic cdc2/cyclin kinase. UV crosslinkage assay demonstrate RNA binding ability of SF2a and RRM domain, but not SF2b protein. Immunostaining of TN-5 cells expressing recombinant proteins and western blot analyzing cytoplasmic and nuclear fractions of expression cells showed targeting of SF2a, SF2b and their SR domains to the nuclei. SR domain, possibly required phosphorylation, is responsible for the nuclear localization signal.
Issue Date:	1998
Type:	Text
Language:	English
Description:	147 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998.
URI:	http://hdl.handle.net/2142/87064
Other Identifier(s):	(MiAaPQ)AAI9904599
Date Available in IDEALS:	2015-09-28
Date Deposited:	1998