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Description
Title: | Molecular Cloning and Structure-Function Analysis of the Plant Proton-Sucrose Symporter |
Author(s): | Lu, Mei-Yeh Jade |
Doctoral Committee Chair(s): | Bush, Daniel R. |
Department / Program: | Plant Biology |
Discipline: | Plant Biology |
Degree Granting Institution: | University of Illinois at Urbana-Champaign |
Degree: | Ph.D. |
Genre: | Dissertation |
Subject(s): | Biology, Cell |
Abstract: | To explore other functionally-important domains, the AtSUC1 cDNA was randomly mutagenized and transformed yeast were screened on sucrose- or maltose-limited media for growth. Three point mutations were identified---H65Q, G334S, and L461P. Significantly, they all displayed enhanced sucrose transport rate. H65Q and G334S also increased maltose transport activity. In the predicted "6-loop-6" topology of the symporter, these residues all face extracellularly, suggesting their involvement in substrate binding. To investigate this hypothesis, the symporter-expressing COS-1 cells were analyzed by epitope-tagging and immunofluorescence. The results showed that both N- and C-termini, as well as the central loop, are located on the cytoplasmic side of the membrane. |
Issue Date: | 1998 |
Type: | Text |
Language: | English |
Description: | 124 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998. |
URI: | http://hdl.handle.net/2142/87067 |
Other Identifier(s): | (MiAaPQ)AAI9912310 |
Date Available in IDEALS: | 2015-09-28 |
Date Deposited: | 1998 |
This item appears in the following Collection(s)
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Dissertations and Theses - Plant Biology
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Graduate Dissertations and Theses at Illinois
Graduate Theses and Dissertations at Illinois