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Title:A Phosphoinositide-Specific Phospholipase C of Trypanosoma Cruzi: Unique Characteristics of a Delta-Type Homologue
Author(s):Furuya, Tetsuya
Doctoral Committee Chair(s):Roberto Docampo
Department / Program:Veterinary Clinical Medicine
Discipline:Veterinary Clinical Medicine
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Microbiology
Abstract:The phosphoinositide-specific phospholipase C gene (TcPI-PLC  ) of the protozoan parasite Trypanosoma cruzi was cloned, sequenced, expressed in E. coli, and the protein product (TcPI-PLC) was shown to have enzymatic characteristics similar to those of mammalian delta-type PI-PLCs. The TcPI-PLC gene is expressed at high levels in the epimastigote and amastigote stages of the parasite, and its expression is induced during the differentiation of trypomastigotes into amastigotes, where it associates to the plasma membrane. In contrast to other PI-PLCs described so far, the deduced amino acid sequence of TcPI-PLC revealed some unique features such as an N-myristoylation consensus sequence at its N-terminal end, lack of an apparent PH domain and a highly charged linker region between the catalytic X and Y domains. TcPI-PLC is lipid modified in vivo as demonstrated by metabolic labeling with [3H]myristate and [3H]palmitate, and immunoprecipitation with anti-TcPI-PLC antibody, and may constitute the first example of a new group of PI-PLCs.
Issue Date:1999
Type:Text
Language:English
Description:119 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999.
URI:http://hdl.handle.net/2142/87142
Other Identifier(s):(MiAaPQ)AAI9953018
Date Available in IDEALS:2015-09-28
Date Deposited:1999


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