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Title:Non-Invasive Detection of Anaerobic Metabolism in Exercising Muscle Using Proton MRI
Author(s):Damon, Bruce Murray
Doctoral Committee Chair(s):Dawson, M. Joan
Department / Program:Molecular and Integrative Physiology
Discipline:Molecular and Integrative Physiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Health Sciences, Radiology
Abstract:The second, and major, component of this thesis concerned the exercise-induced increases the 1H T2 of muscle water. The hypotheses were tested that the T2 increase results from increased intracellular volume (VI), decreased intracellular pH (pHI), and/or changes in magnetization transfer (MT) or other longitudinal relaxation mechanisms. Isolated frog gastrocnemii were treated with iodoacetate (IAA; to inhibit lactate production), NaCN (to maximize lactate production), or left untreated and then performed 60 1s/min maximal isometric contractions. The intracellular transverse relaxation time (T2,I increased more in NaCN-treated and untreated muscles than in IAA-treated muscles. The VI increase and pHI decrease were greater in NaCN-treated and untreated muscles than in IAA-treated muscles. There was no change in the MT ratio and a slight decrease in T1 following exercise. In separate experiments, V I and pHI were manipulated in non-exercising sartorii and quantitative relationships were developed between these variables and T 2,I. A multiple regression model shows that 62% of the variance in the T2,I change of exercise is accounted for by these two variables.
Issue Date:2000
Description:210 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000.
Other Identifier(s):(MiAaPQ)AAI9971061
Date Available in IDEALS:2015-09-28
Date Deposited:2000

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