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Title:Fatty Acid-Mediated Inhibition of Host Cell Invasion by Cryptosporidium Parvum
Author(s):Schmidt, Jo Ann
Doctoral Committee Chair(s):Kuhlenschmidt, Mark S.
Department / Program:Veterinary Pathobiology
Discipline:Veterinary Pathobiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Microbiology
Abstract:We have purified from bovine colostrum digests an unsaturated fatty acid that inhibits Cryptosporidium parvum sporozoite invasion of host cells in vitro. A panel of similar lipids with varying chain lengths and degrees of saturation were tested for evidence of inhibitory activity in order to determine the range of specificity. We identified a subset of inhibitory fatty acids and investigated the structural characteristics required for anti-cryptosporidial activity. Finally, we examined potential mechanisms for the lipid-mediated inhibition of sporozoite-host cell adhesion and invasion in vitro. These mechanisms included direct cytotoxicity to host cells and/or parasites, interruption of normal signaling pathways and alterations in gene expression. Our assays showed that eighteen-carbon unsaturated and twenty-carbon polyunsaturated free fatty acids with free carboxyl ends inhibited the invasion of host enterocytes by sporozoites in vitro by blocking microneme secretion. These results indicate a potential chemotherapeutic utility for a subset of long chain unsaturated free fatty acids in the treatment of cryptosporidiosis.
Issue Date:2005
Description:183 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.
Other Identifier(s):(MiAaPQ)AAI3199135
Date Available in IDEALS:2015-09-28
Date Deposited:2005

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