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Title:Mechanistic and synthetic studies on the prochlorosin and cytolysin families of lanthipeptides
Author(s):Mukherjee, Subha
Director of Research:van der Donk, Wilfred Adrianus
Doctoral Committee Chair(s):van der Donk, Wilfred Adrianus
Doctoral Committee Member(s):Burke, Martin D.; Metcalf, William W.; Zhao, Huimin
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):ribosomally synthesized and post-translationally modified peptides (RiPPs)
Lanthipeptide
Lanthionine
antibiotics
antimicrobial resistance
prochlorosin
ProcM
Solid phase peptide synthesis (SPPS)
lanthipeptide synthesis
hydrophobic peptide synthesis
dehydroalanine
Michael-type addition
non-enzymatic cyclization
cytolysin
microbisporicin
phosphonate
dehydrophos
fosfazinomycin
rhizocticin
Abstract:Peptides are an attractive class of therapeutics, occupying a niche between small molecules and biologics. Research in the van der Donk lab focuses on lanthipeptides, a class of ribosomally synthesized and post-translationally modified peptides (RiPPs) that commonly feature antibacterial activity and contain the characteristic thioether residues lanthionine (Lan) and methyllanthionine (MeLan). Installation of thioether crosslinks in lanthipeptide biosynthesis is carried out by designated synthetases and involves dehydration of Ser/Thr residues and cyclization via Michael-type addition. The remarkably broad substrate scope of the synthetase ProcM inspired us to explore its mechanism in detail (chapter 2). My studies on ProcM revealed the directionality of dehydration, the order of cyclization, and that, despite the impressive substrate scope, none of the cyclizations are non-enzymatic. In collaboration, we established the irreversibility of the Michael-type addition and proposed that the topology of the thioether rings is under kinetic control. Solid phase peptide synthesis (SPPS) was used to generate the substrates to study ProcM, and is also a flexibile tool to access non-native lanthipeptide analogues. Interestingly, a lanthipeptide, cytolysin S (CylLS”), exhibited cytolytic activity in synergy with cytolysin L (CylLL”). Given that a thioether crosslink in CylLS” has an unusual LL-stereochemistry, the synthesis of a diastereomer of CylLS” with the more common DL-stereochemistry was achieved by SPPS (chapter 3). We probed whether the cytolytic activity depended on the LL-stereochemistry observed in CylLS”. Surprisingly, the unusual LL-stereochemistry was found to be important for the antibacterial activity, but not necessary for the hemolytic activity of CylLS”. I have also synthesized another hydrophobic lanthipeptide analogue, the portion of microbisporicin that contains the A and B ring (chapter 4). We established that this motif is not recognized by the halogenase MibH, and that the C terminus of microbisporicin is necessary for the chlorination by MibH. During my graduate studies, I had the opportunity to collaborate in a different area of research in our laboratory, the phosphonates. My efforts in the syntheses of various substrates and intermediates were instrumental in elucidating the biosynthetic pathways of dehydrophos, fosfazinomycin, and rhizocticin (chapter 5).
Issue Date:2015-09-17
Type:Thesis
URI:http://hdl.handle.net/2142/89174
Rights Information:Copyright 2015 Subha Mukherjee
Date Available in IDEALS:2016-03-02
Date Deposited:2015-12


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