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Title:Femtosecond pulse shaping for enhanced optical imaging and control of cells
Author(s):Ark, Eugene D
Advisor(s):Boppart, Stephen A.
Department / Program:Electrical & Computer Engineering
Discipline:Electrical & Computer Engineering
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Femtosecond pulse shaping
caged compound
Abstract:Optical techniques have garnered increasing interest in biomedical research. As the types of optical probes available continue to expand, the appeal of experiments utilizing multiple probes simultaneously grows rapidly. Unfortunately, the significant spectral overlap in probe responsiveness presents limitations on their potential applications. Much of the effort in circumventing this issue has been focused on developing modified probes with shifted spectral sensitivities; however, this is a long and inefficient process that tends to have undesired consequences on probe activity. This thesis explores the possibility of using temporal pulse shaping in order to improve the selectivity with which optically sensitive molecules can be activated. It is demonstrated that linearly chirped pulses and dark pulses have the potential to improve selectivity by exclusively controlling the spectral phase function of the illumination source for three classes of optical probes: fluorescent molecules, caged compounds, and opsins.
Issue Date:2015-11-24
Rights Information:Copyright 2015 Eugene D. Ark
Date Available in IDEALS:2016-03-02
Date Deposited:2015-12

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