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Title:Putative regulatory role of GlyS antisense RNA in an obligate insect symbiont Buchnera aphidicola
Author(s):Cheng, Siyuan
Contributor(s):Hansen, Allison K.
Subject(s):small RNA, obligate insect symbiont, gene regulation, bacteria, Buchnera aphidicola
Abstract:My research seeks to answer the question of how small RNAs regulate the gene expression in an uncultivable obligate insect symbiont Buchnera aphidicola, which is important for deeper understanding of the influence of gene regulation on host-symbiont interaction and co-evolution. This presentation will discuss how I apply the novel dual plasmid vector system to the investigation of an uncultivable symbiont gene regulation in vivo. Thus far, the plasmids encoding the antisense RNA (asRNA) of a candidate gene (glyS) has been constructed and transformed into E. coli cells. Next, the DNA coding sequence (CDS) of glyS will be amplified and restrict-digested. This will enable the other plasmids to be constructed with the CDS and transformed into E. coli cells. The activation or inhibition of the gene expression by the asRNA will be measured with the green fluorescent protein (GFP) that is fused with the CDS. The research would lead to more insights on how small RNAs regulate the gene expression of bacteria with reduced genome in the absence of transcription factors and operons. These insights would help us understand the mechanisms of gene regulation in bacteria, which would decipher the genome co-evolution of hosts and symbionts.
Issue Date:2016
Citation Info:Cheng, Siyuan. (2016, April). "Putative regulatory role of GlyS antisense RNA in an obligate insect symbiont Buchnera aphidicola" session presented at Undergraduate Research Symposium, University of Illinois at Urbana-Champaign, Illinois
Genre:Conference Poster
Type:Text
Language:English
URI:http://hdl.handle.net/2142/90105
Rights Information:Copyright 2016, Cheng
Date Available in IDEALS:2016-05-18


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