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Title:Effect of soil on the mutagenic properties of waste water
Author(s):Plewa, Michael J.; Hopke, Philip K.
Contributor(s):University of Illinois at Urbana-Champaign
Subject(s):Water resource development
Water resource development--Illinois
Public health
Salmonella typhimurium
Tradescantial waste water
Zea mays
Geographic Coverage:Illinois (state)
Abstract:The disposal of complex mixtures such as waste water on agricultural lands poses known and unknown environmental risks. Mutagens may be introduced into the eco-system and perhaps concentrated by crop plants or leached into ground water supplies. The purpose of this study was to determine the biological effect of a mutagenic waste water before and after application to soil. We used an XAD-8 methanol extract of waste water from the municipal sewage treatment facility at Sauget IL. This extract was a potent direct acting mutagen when assayed with the Salmonella typhimurium. 1 and 3 ml of extract were brought up to 10 ml volumes with water and added to 10 a of sterile or nonsterile , native clay loam. These mixtures were placed in a shaking water bath at room temperature for 0, 24, and 48 h. After separation of solid and liquid portions by filtration, dichloromethane was added to extract the organic fractions from each component. Solvent extractions were evaporated to dryness under vacuum and brought up in DMSO. Tests for mutagenic activity were conducted using strain TA98. After addition to the soil for greater periods of time the mutagenic activity decreased. The solid component exhibited greater mutagenic activity than the liquid. The XAD-8 extract was also assayed using the yg2 assay in Zea mays and the micronucleus assay in Tradescantia. The extract did not induce mutation or chromosome aberrations in these assays. The sludge from the Sauqet plant was chemically fractionated and assayed with S. typhimurium strains TA98 and TA100. The neutral fraction was the most mutagenic fraction followed by the weak acid,-strong acid and basic fractions. These chemical fractions uncovered more mutagenic potency than was predicted by assaying a crude organic extract of the sludge.
Issue Date:1984-12
Publisher:University of Illinois at Urbana-Champaign. Water Resources Center
Genre:Report (Grant or Annual)
Sponsor:U.S. Department of the Interior
U.S. Geological Survey
Rights Information:Copyright 1984 held by Michael J. Plewa, Philip K. Hopke
Date Available in IDEALS:2016-05-25

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