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Title:Analysis of the ability of Staphylococcus aureus to utilize glycerol-3-phosphate as a phosphate source
Author(s):Grudzinski, Kevin; Kelliher, Jessica
Contributor(s):Kehl-Fie, Thomas
Subject(s):staphylococcus aureus
microbial physiology
Abstract:Staphylococcus aureus is a devastating pathogen that colonizes a third of the population. Multidrug-resistant strains continue spreading, leading the CDC to state that S. aureus is a serious threat to public health. During infection, pathogens must obtain all of their nutrients from the host. Transporters dedicated to the acquisition of phosphate, an essential nutrient, have been implicated in the virulence of other pathogens. However, how S. aureus obtains phosphate is unknown. Answering this question will enhance our understanding of staphylococcal disease. In the host, phosphate can be found as inorganic phosphate and organophosphates, such as glycerol-3-phosphate (G3P). Analysis of the staphylococcal genome identified a putative G3P transporter. Subsequently, we found that G3P can be used as a phosphate source by S. aureus. A strain lacking a putative alkaline phosphatase, PhoB, grows similarly to wild type in the absence of glucose when G3P is the only phosphate source, suggesting this molecule can be imported whole into the cell. However, transcriptional analysis revealed that the importer, GlpT, is not induced by phosphate starvation, suggesting phosphate acquisition may not be its primary role. In the presence or absence of glucose, a glpT mutant grew similar to wild type when G3P was the sole phosphate source. However, a phoB mutant was severely attenuated in the presence of glucose with G3P as the sole phosphate source. Cumulatively, our data suggests S. aureus preferentially utilizes PhoB to cleave G3P extracellularly, importing glycerol and phosphate separately under phosphate or carbon starvation.
Issue Date:2016
Citation Info:Grudzinski, Kevin and Jessica Kelliher. (2016, April). "Analysis of the ability of Staphylococcus aureus to utilize glycerol-3-phosphate as a phosphate source" session presented at Undergraduate Research Symposium, University of Illinois at Urbana-Champaign, Illinois
Genre:Conference Poster
Sponsor:NIH K22 (AI 104805) and a March of Dimes Basil OConnor Starter Scholar Research Award
Rights Information:Copyright 2016, Gruzindski
Copyright 2016, Kelliher
Date Available in IDEALS:2016-06-09

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