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Title:Selective isolation of dissolved organic matter from aquatic systems
Author(s):John R. Tuschall, Jr.; George, Gregory
Contributor(s):Illinois State Water Survey
Subject(s):Water resource development--Illinois
Water resource development
Water quality
Amino acids
Analytical techniques
Anion exchange
Cation exchange
Chemical properties
Decomposing organic matter
Dissolved organic matter
Fulvic acids
Humic acids
Liquid chromatography
Organic matter
Separation techniques
Surface waters
Water chemistry
XAD resins
Geographic Coverage:Illinois (state)
Abstract:A chromatographic ion-exchange procedure for fractionation, isolation and concentration of dissolved organic matter (DOM) in surface waters was developed and applied to samples from Illinois lakes. Isolation of the acidic (humic) DOM fraction was optimized using solutions of simple model compounds chemically similar to humics and commercially available soil humic acid. Recoveries of DOM from five humic-containing (colored) surface waters were in excess of 90%. A series of experiments using model compounds, various desorption solutions, and select charge modified and unmodified cellulose separation materials demonstrated that the mechanism of sorption of the acidic fraction, and of its recovery by controlled desorption, is simple ion exchange. Hydrophobic (nonpolar) interactions did not affect sorption processes as has been suggested by other authors. Subsequently, filtered water samples were applied to a fractionation scheme designed to separate the DOM into anion exchanged (acidic), cation exchanged (basic) and nonexchanged (neutral) fractions. Of the DOM, most of the dissolved organic carbon and a large portion of dissolved organic nitrogen (DON) in all samples was recovered in the acidic fraction, with the neutral fraction containing most of the remaining DOM. The cationic (at pH 3) fraction contained a lower portion of the DOM than expected, indicating that DON in the water samples was not composed of positively charged amines available for ion exchange under the experimental conditions. DON was monitored throughout the fractionation scheme by a sensitive fluorescence (o-phthaldialdehyde) method developed in this study and generally applicable to measuring small samples containing low concentrations of DON found in natural waters. The lack of suitable methods for isolating and concentrating dissolved organic matter has hindered progress on its chemical characterization. The fractionation scheme developed and tested is a useful tool for researchers studying the chemical nature of dissolved organic matter, which affects water quality in many ways.
Issue Date:1984-06
Publisher:University of Illinois at Urbana-Champaign. Water Resources Center
Genre:Report (Grant or Annual)
Sponsor:U.S. Department of the Interior
U.S. Geological Survey
Rights Information:Copyright 1984 held by John R. Tuschall, Jr.,Gregory George
Date Available in IDEALS:2016-06-29

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