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Title:Investigating the epidemiology of Terrapene herpesvirus 1 in freeranging eastern box turtle populations
Author(s):Kane, Lauren P
Department / Program:Comparative Biosciences
Discipline:VMS - Comparative Biosciences
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:M.S.
Genre:Thesis
Subject(s):Epidemiology
herpesvirus
Eastern box turtle
qPCR
Terrapene carolina
disease
Abstract:Herpesviruses are ubiquitous, well described pathogens in captive chelonians worldwide, but their importance on free-ranging populations are less defined. In this thesis, a quantitative PCR was developed that detected a 58 base pair segment of the DNA polymerase gene segment of Terrapene herpesvirus 1 (Order: Herpesvirales; Family: Herpesviridae; Subfamily: Alphaherpesvirinae; Genus: Scutavirus). This assay was used to estimate prevalence of herpesvirus infection in 409 free-ranging eastern box turtles (Terrapene carolina carolina) from Tennessee and Illinois. The overall prevalence in this study population was 31.3% (95% confidence interval: 27-36%), with a significantly higher prevalence in July (52.3%; 95% CI: 41-59%) compared to May (13.3%; 95% CI: 5-15%) and September (34.4%; 95% CI: 29-47%). Clinical signs recorded in box turtles were not significantly associated with herpesvirus infection and may be attributed to a latency period. The work presented in this thesis aids in characterizing the epidemiology of herpesvirus in chelonians.
Issue Date:2016-03-18
Type:Thesis
URI:http://hdl.handle.net/2142/90487
Rights Information:Copyright 2016 LAUREN P. KANE
Date Available in IDEALS:2016-07-07
Date Deposited:2016-05


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