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Title:The Role of the eIF3 complex in Specialized Transcript Specific Translation
Author(s):Willis, Alexander B.
Contributor(s):Jin, Hong
protein purification
Abstract:Translational initiation is a complex process in eukaryotes that necessitates a multitude of proteins known as initiation factors (eIFs). eIF3, a protein whose core structure is conserved throughout eukaryotes, plays a number of key roles in the translational process including binding free 40S ribosome and serving as a scaffold for other initiation factor binding, aiding in scanning for the start codon, assisting in ribosome recycling, and importantly binding certain mRNA structures in 5’ UTRs to promote cap independent translation. In these studies, the six-subunit eIF3 complex was purified from chromosomally eIF3b-TAP-tagged saccharomyces cerevisiae(Tandem Affinity Chromatography). This complex will be used in addition with other initiation factors to perform translation initiation assays on mRNAs identified via high throughput screening as being candidates for non-canonical transcript specific translation.
Issue Date:2017
Genre:Dissertation / Thesis
Rights Information:Copyright 2017 Alexander B. Willis
Date Available in IDEALS:2017-05-16

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