Computer-interfaced, Servo-actuated Microfluidic Control System for Single Cell Analysis

Abstract

Microfluidics and "lab-on-chips" are currently major areas of research. Integrated chips provide many advantages over the current macroscopic methods in biological applications such as increased throughput, better automation, reduced reagent consumption, increased surface/volume ratios and stable laminar flows. Unfortunately, the control of flows in microchannels has special challenges, and conventional flow control tools such as syringes or peristaltic pumps are generally poorly adapted. In order to facilitate the further development of a lab-on-chip system for single cell analysis, a special control system based on hydrostatic pressure is used. The system is compact, portable, inexpensive, and computer-interfaced for precise control and ease of use. The level of fluid in various reservoirs is changed by a computer-controlled servo to create pressure differences. The change in fluid level causes flow through the channels in the chip. A detailed description of the system is presented. Further development of the system is possible and could lead to many improvements and adaptations for specific applications.