Files in this item

FilesDescriptionFormat

application/pdf

application/pdf2625.pdf (17kB)
(no description provided)PDF

Description

Title:S-NITROSOTHIOLS OBSERVED USING CAVITY RING-DOWN SPECTROSCOPY
Author(s):Rad, Mary Lynn
Contributor(s):Lehmann, Kevin; Gaston, Benjamin M
Subject(s):Spectroscopy as an analytical tool
Abstract:The biological importance of nitric oxide has been known for nearly forty years due to its role in cardiovascular and nervous signaling. The main carrier molecules, s-nitrosothiols (RSNOs), are of additional interest due to their role in signaling reactions. Additionally, these compounds are related to several diseases including muscular dystrophy, stroke, myocardial infarction, Alzheimer's disease, Parkinson's disease, cystic fibrosis, asthma, and pulmonary arterial hypertension. One of the main barriers to elucidating the role of these RSNOs is the low (nanomolar) concentration present in samples of low volume (typically $sim$100 $mu$L). To this end we have set up a cavity ring-down spectrometer tuned to observe $^{14}$NO and $^{15}$NO released from cell growth samples. To decrease the limit of detection we have implemented a laser locking scheme employing Zeeman modulation of NO in a reference cell and have tuned the polarization of the laser using a half wave plate to optimize the polarization for the inherent birefringence of the CRDS mirrors. Progress toward measuring RSNO concentration in biological samples will be presented.
Issue Date:6/21/2017
Publisher:International Symposium on Molecular Spectroscopy
Citation Info:APS
Genre:CONFERENCE PAPER/PRESENTATION
Type:Text
Language:English
URI:http://hdl.handle.net/2142/96993
DOI:10.15278/isms.2017.WE03
Date Available in IDEALS:2017-07-27


This item appears in the following Collection(s)

Item Statistics