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Title:Discovery and structural characterization of lanthipeptides from soil and rumen bacteria
Author(s):Zhao, Xi Ling
Director of Research:van der Donk, Wilfred A.
Doctoral Committee Chair(s):van der Donk, Wilfred A.
Doctoral Committee Member(s):Metcalf, William W.; Mitchell, Douglas A.; Sweedler, Jonathan V.
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Ribosomally synthesized and post-translationally modified peptide natural products (RiPPs)
Lanthipeptides
Heterologous expression of gene clusters
Combinatorial biosynthesis
Abstract:As members of the ribosomally synthesized and post-translationally modified peptide natural products (RiPPs), lanthipeptides possess myriad structural diversity and potential for discovery using genome-guided approaches. Biological activities of interest embedded within lanthipeptide structures include antimicrobial, antiviral, and morphogenetic functions. Continued discovery efforts seek to expand the characterized activities and structures of these compounds, as well as the enzymes that execute their biosynthesis. The contents of this thesis are focused on the discovery of lanthipeptides using a variety of methods. Chapters 2 and 4 demonstrate the application of heterologous expression of gene clusters to produce lanthipeptides. The resulting peptide structures and enzyme activities provide intriguing insights into diversity of biosynthetic approaches present in this class of compounds. Furthermore, the abundance of precursor peptides encoded in the lanthipeptide biosynthetic systems discussed in Chapters 2 and 4 offer examples of combinatorial biosynthesis and the tantalizing possibility to harness this attribute. In contrast, Chapter 3 provides a glimpse into the targeted screening of Actinobacteria for the production of lanthipeptides, which suggests additional structural diversity. The successful application of these methods increases the number of lanthipeptide compounds and systems, which ultimately allows for a deeper understanding of and appreciation for this class of natural products.
Issue Date:2017-02-17
Type:Thesis
URI:http://hdl.handle.net/2142/97537
Rights Information:Copyright 2017 Xi Zhao
Date Available in IDEALS:2017-08-10
Date Deposited:2017-05


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