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Title:A study on the modulation of alpha-actinin and filamentous actin
Author(s):Kemp, James Peter
Director of Research:Brieher, William M
Doctoral Committee Chair(s):Brieher, William M
Doctoral Committee Member(s):Freeman, Brian C; Gillette, Martha U; Saif, Taher A
Department / Program:Cell & Developmental Biology
Discipline:Cell and Developmental Biology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Stress fibers, alpha-actinin
Abstract:Cells use actin to provide structure and stability to the membrane, to drive motility, and to adhere to, pull on, and reorganize the extracellular matrix. Here I demonstrate that α-actinin-4, a well-known actin crosslinking protein and long believed to be the primary actin bundling protein for contractile actin arrays, actually inhibits bundling of actin filaments into stress fibers in epithelial cells. I show that α-actinin-4 accomplishes this by inhibiting tropomyosin dependent stabilization of filamentous actin, and maintains a dynamic basal actin array through promoting actin disassembly. Lastly I demonstrate that α-actinin-4 localization in cells can be modulated by phosphorylation of its actin-binding domain.
Issue Date:2017-04-14
Type:Thesis
URI:http://hdl.handle.net/2142/97561
Rights Information:Copyright 2017 James Peter Kemp Jr
Date Available in IDEALS:2017-08-10
Date Deposited:2017-05


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