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Title:Noise in noise out - single molecule studies of molecular motors
Author(s):Tjioe, Marco
Director of Research:Selvin, Paul R
Doctoral Committee Chair(s):Selvin, Paul R
Doctoral Committee Member(s):Chemla, Yann R; DeMarco, Brian L; Zhang, Kai
Department / Program:School of Molecular & Cell Bio
Discipline:Biophysics & Computnl Biology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Single molecule, kinesin, dynein, noise, frames per step (FPS), magnetic purification, signal-to-noise ratio, molecular motor
Abstract:Many cellular functions rely on the movements of molecular motors. Single molecule studies of these motors allow investigation into their step-sizes and stall forces. Such studies inevitably involve noise, which is inherent in the biological and imaging systems probing these motors. Having to work with noise in the system, we develop a parameter, called the FIONA Index, which serves as a guide for data quality based on noise per step-size (NPS) and frames per step (FPS). Attempting to bring the noise out of the system, we develop Magnetic Cytoskeleton Affinity (MiCA) purification to remove excess dyes that prevent clear observation of individual motors. We show that with appropriate treatment of noise, complex systems can be assembled to study how multiple motors work together.
Issue Date:2017-05-26
Type:Thesis
URI:http://hdl.handle.net/2142/98156
Rights Information:Copyright 2017 Marco Tjioe
Date Available in IDEALS:2017-09-29
Date Deposited:2017-08


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