Files in this item
| Files | Description | Format |
|---|---|---|
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application/pdf  TJIOE-DISSERTATION-2017.pdf (5MB) | (no description provided) |
Description
| Title: | Noise in noise out - single molecule studies of molecular motors |
| Author(s): | Tjioe, Marco |
| Director of Research: | Selvin, Paul R |
| Doctoral Committee Chair(s): | Selvin, Paul R |
| Doctoral Committee Member(s): | Chemla, Yann R; DeMarco, Brian L; Zhang, Kai |
| Department / Program: | School of Molecular & Cell Bio |
| Discipline: | Biophysics & Computnl Biology |
| Degree Granting Institution: | University of Illinois at Urbana-Champaign |
| Degree: | Ph.D. |
| Genre: | Dissertation |
| Subject(s): | Single molecule
Kinesin Dynein Noise Frames per step (FPS) Magnetic purification Signal-to-noise ratio Molecular motor |
| Abstract: | Many cellular functions rely on the movements of molecular motors. Single molecule studies of these motors allow investigation into their step-sizes and stall forces. Such studies inevitably involve noise, which is inherent in the biological and imaging systems probing these motors. Having to work with noise in the system, we develop a parameter, called the FIONA Index, which serves as a guide for data quality based on noise per step-size (NPS) and frames per step (FPS). Attempting to bring the noise out of the system, we develop Magnetic Cytoskeleton Affinity (MiCA) purification to remove excess dyes that prevent clear observation of individual motors. We show that with appropriate treatment of noise, complex systems can be assembled to study how multiple motors work together. |
| Issue Date: | 2017-05-26 |
| Type: | Text |
| URI: | http://hdl.handle.net/2142/98156 |
| Rights Information: | Copyright 2017 Marco Tjioe |
| Date Available in IDEALS: | 2017-09-29 |
| Date Deposited: | 2017-08 |
This item appears in the following Collection(s)
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Dissertations - Biophysics and Computational Biology
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Graduate Dissertations and Theses at Illinois
Graduate Theses and Dissertations at Illinois