|Abstract:||The objective of the first study was to evaluate the nutritional value of Glutenol™, a new coproduct of the ethanol industry. Glutenol™ was produced by Quality Technology International in a modified wet-milling plant using a hybrid process, NextGenFrac™, which fractionates the corn kernel components prior to fermentation without the use of sulfur dioxide. Glutenol™ was analyzed to contain 52.3% CP, 1.7% Met + Cys, 1.32% Lys, 1.69% Thr, and 2.23% Val on a DM basis. Two precision-fed rooster assays with conventional and cecectomized roosters were conducted to determine TMEn and amino acid digestibility, respectively. The TMEn of Glutenol™ was determined to be 3256 kcal/kg DM. Standardized digestibility values for Lys, Met, Cys, Thr and Val were 80.1%, 90.4%, 74.1%, 81.1% and 84.9% respectively. In addition, a three-week broiler chick assay was conducted with increasing levels of dietary Glutenol™. Diet 1 was a standard corn/soybean meal diet with 0% Glutenol™. Diets 2, 3 and 4 had increasing levels of Glutenol™ at 4%, 8% and 12% respectively. As the level of Glutenol™ increased, the level of SBM in the diet decreased from 35.77% in Diet 1 to 29.42% in Diet 4 to keep the protein levels equal among treatments. The diets were fed to Ross 308 male broilers housed in battery cages from 3 to 22 days post hatch. All diets were formulated to be equal in ME and digestible amino acids. Weight gain, feed intake and gain/feed ratio were measured. No significant differences in growth performance were observed among dietary treatments, leading to the conclusion that Glutenol™ can be fed up to at least 12% in the diet of broiler chickens if diets are formulated to be equal in ME and digestible amino acids. In the second study, a series of 48-hour precision-fed rooster assays were conducted to determine TMEn using conventional roosters and/or amino acid digestibility using cecectomized roosters for distiller’s dried grains with solubles (DDGS) produced from human food waste at high solids content (FWDDGS), DDGS produced from two mutant corn hybrids containing either increased protein or increased levels of select dietary indispensable amino acids such as lysine, arginine, and tryptophan, and an increased-protein ethanol co-product that is produced by a process which separates a high concentration protein and yeast fraction from ethanol stillage (Still Pro™, Fluid Quip Process Technologies, Cedar Rapids, IA). The original raw materials for the FWDDGS consisted mainly of, mashed potatoes, sweet corn, and white bread. The results of the rooster assays indicated that the digestibility of the amino acids in the FWDDGS was similar to typical DDGS (e.g., 61% for lysine), whereas the TMEn for the FWDDGS was higher than for typical DDGS. The DDGS produced from the high protein mutant corn hybrid was shown to have a higher protein content of 34% compared with DDGS from its control corn hybrid, which contained 28% protein. Using Lys as an example, there was a large difference in Lys content between the two samples; the high protein mutant DDGS contained 1.60% Lys versus 1.05% for the control DDGS. The digestibility of amino acids was generally similar for the mutant DDGS and the control DDGS. Similar results were observed for Lys, Arg, and Trp for the DDGS produced from the second mutant corn hybrid. The Still Pro™ sample was analyzed to contain 53% protein on a dry matter basis with 2.22 % Lys, 1.05% Met, 0.90% Cys, and 2.06% Thr. The digestibility values for these amino acids were 84%, 92%, 87%, and 86% respectively. The TMEn of the Still Pro™ sample was determined to be 3372 kcal/kg on a dry matter basis. The results of the two studies indicate that the nutritional value of the four novel ethanol coproducts is generally superior to the nutritional value of conventional DDGS.