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Title:Discovery of Glycocin from Pathway Refactoring
Author(s):Ho, Sherri; Ren, Hengqian
Contributor(s):Zhao, Huimin
Subject(s):Molecular and Cellular Biology
Synthetic Biology
Natural Product
Protein Purification
Abstract:Natural products have been playing an indispensable role in pharmaceuticals and human therapeutics for decades. However, because of the continuous development of drug resistance, high throughput discovery of natural products is always an urgent but seemingly impossible challenge. Synthetic biology, with its power to engineer biosynthetic pathways, has proven to be a promising solution to this issue. One type of natural product that will be investigated are ribosomally synthesized and post-translationally modified peptide (RiPP) natural products. Glycocins, a class of RiPPs, are a form of bacterial toxins that can inhibit the growth of many bacterial strains. In this study, we used heterologous expression to engineer the pathway of glycocin from Geobacillus followed by fermentation, fractionation, and purification of the glycocin. In the end, we discovered a glycocin from the thermophilic bacteria Geobacillus which has never been done before.
Issue Date:2018-04
Genre:Conference Poster
Sponsor:MCB SROP
Rights Information:Copyright 2018 Sherri Ho
Copyright 2018 Hengqian Ren
Date Available in IDEALS:2018-05-21

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